Activists threw soup at Claude Monet's "Le Printemps" (Spring) at 3:30 p.m. local time on Saturday, according to a statement from the Musee des Beaux-Arts in France's third largest city.
It was the latest action by a campaign group that carried out a similar action against the Mona Lisa last month.
The museum said the 1872 painting is protected by glass but will still undergo a close inspection and restoration.
The 1872 painting was protected by glass, but will still undergo a close inspection and restoration, the museum said.
The museum said it would file a complaint for vandalism, adding that two activists were arrested.
Riposte Alimentaire ("Food counterattack") claimed the attack in a posting on X, with a woman identifying herself 20-year-old Ilona saying "we have to act now before it is too late."
The same group, which calls for a sustainable supply of healthy food for all, also claimed January's soup attack on the Louvre museum's Mona Lisa painting, which was also behind glass.
The two militants who carried out the attack on Leonardo da Vinci's iconic work were condemned by a Paris court to carry out volunteer work for a charity association.